Hundreds more survivors and dozens more bodies still underground, according to a miners rights group.
The move escalates the case against Kizza Besigye as a treachery conviction is punishable by the death penalty.
Attorney General Tarek Saab says he has appealed to Interpol for the arrest of Leopoldo Lopez, who lives in exile.
With 15 inmates remaining, advocates urge Biden to release more Gitmo detainees before he leaves office on January 20.