Protests, now in their 12th week, built up over the summer as Netanyahu fights off corruption case amid the pandemic.
Thousands of Israelis have protested across the country for the 15th week in a row, flouting a new law meant to curb anti-government demonstrations during a coronavirus lockdown.
The street protests on Saturday and into Sunday, just three days after parliament approved an edict to limit the scope of such demonstrations, kept pressure on Prime Minister Benjamin Netanyahu to resign for his handling of the coronavirus crisis, as well as allegations of corruption, which he denies.
Netanyahu is on trial for bribery, fraud and breach of trust. He has denied wrongdoing.
Israeli law does not force a prime minister to step down after being indicted, sparking outrage and protests at a time when the country also grappled with the pandemic.
The new law bans Israelis from holding demonstrations more than one kilometre (about half a mile) from their homes and forces stricter social distancing, a measure the government said was aimed at curbing COVID-19 infections.
Critics have called it a blow to freedom of speech.
According to protest organisers, an estimated 100,000 people rallied in squares and at junctions throughout the country from sunset on Saturday.
A small number of protesters scuffled with police and tried to block city streets.
According to news reports, more than 30 people were arrested in Tel Aviv by police.
Israel has shut down much of its economy and instructed people to stay within a kilometre of their homes whenever possible in an effort to contain a second-wave surge in coronavirus infections.
As of Sunday, Israel has recorded more than 264,000 infections, out of which more than 191,000 have recovered, while the death toll has hit almost 1,700 out of a population of nine million.