Six men killed in missile attack on their vehicle in central Gaza.
Israel has carried out a number of operations inside Gaza since Hamas took full control of the territory in June.
The three men were taken to the Nasir hospital morgue in Khan Yunis.
An Islamic Jihad spokesman said the group would continue firing rockets into Israel.
West Bank raid
Earlier on Tuesday, a Palestinian fighter was shot dead in clashes with Israeli soldiers in the al-Ein refugee camp in the West Bank city of Nablus.
Nasir Mabrouk belonged to the Abu Ali Mustafa Brigades, the armed wing of the Popular Front for the Liberation of Palestine.
The Israeli operation in the camp, which started two days ago, has included the arrest of a number of people living there and the searching of many buildings.
On Monday, six Hamas militants were killed in an Israeli air strike on a jeep in the el-Bureij refugee camp in central Gaza.
The latest deaths took to 5,831 the number of people killed in Israeli-Palestinian violence since the start of the second Palestinian uprising in 2000, the vast majority of them Palestinian.