They won the tournament in 2012, but it is looking increasingly unlikely Chelsea will get through to the last 16 of the Champions League after suffering a 3-0 defeat to Juventus on Tuesday.
In the earlier Champions League matches, Barcelona qualified for the next stage with victory over Spartak Moscow and Lille defeated BATE Borisov.
Juventus' fate is now in their own hands with a victory taking them through to the next stage.
Fabio Quagliarella and Arturo Vidal - who were both on target for Juventus in the 2-2 draw at Stamford Bridge - again scored against Chelsea. Substitute Sebastian Giovinco sealed the result in the final minute.
Juventus are second in Group E, two points above Chelsea and one behind Shakhtar Donetsk, who they face in the last group match. A draw in Ukraine would allow both teams to progress.
If that should happen, Chelsea will become the first reigning champions to fail to make it out the group stages. The Blues have also been on a poor run in the English Premier League without a win in four-games.
In the other Group E game, Shakhtar Donetsk kept their lead thrashing Nordsjaelland 5-2.
Celtic's chances of qualifying look a bit healthier than Chelsea's despite suffering a 2-1 defeat to Benfica.
The result left the Portuguese and Scottish teams level on seven points.
Group leaders Barcelona have 12 points and Spartak Moscow three. The deciding group matches on December 5 have Benfica travelling to Barcelona and Celtic hosting Spartak.
Manchester United suffered a 1-0 defeat at Galatasaray but boss Alex Ferguson won't be too worried as his side are already through to the last 16.
Burak Yilmaz headed in from a corner at the start of the second half and has scored all five goals in the competition for Galatasaray.
"I think they handled the atmosphere and the temperament was good," Ferguson said.
"They handled that OK. Parts of the game were good but the area in which we were worried about - set-pieces - we suffered. That was always going to be a worry for us.
"Other than that, I was satisfied and it was a decent performance. We had some good attacking play in the first half and at that point the only trouble they gave us was from outside the box."
Turkish side Galatasaray and CFR Cluj now will do battle for the final space in Group H with both teams on seven points.
Cluj defeated Braga 3-1 with a hat-trick from Rui Pedro.
"We still want to finish first in the group so winning our last match is vital"
Valencia's Sofiane Feghouli
In the last round of group matches, Cluj visit United while Galatasaray go to the eliminated Braga.
Valencia and Bayern Munich are both through to the next stage after a 1-1 draw between the sides.
The Spaniards went down to 10 men in the 33rd minute after right back Antonio Barragan fouled David Alaba to receive a second yellow card. Despite the disadvantage, Valencia midfielder Sofiane Feghouli made it 1-0 in the 77th minute with a bouncing shot into the net.
Bayern winger Thomas Mueller equalised from a loose ball in the penalty area that grazed Feghouli and went into the net in the 82nd minute.
"It was a fantastic performance from us," said Feghouli.
"Although we wanted to win for our fans, we will leave here satisfied.
"We still want to finish first in the group so winning our last match is vital."
Bayern and Valencia both have 10 points, an uncatchable-four ahead of BATE Borisov and seven in front of Lille.