Rodriguez, who earlier this week signed a record multi-million dollar contract with New York, insisted he has never used performance-enhancing drugs when quizzed on the American news programme 60 Minutes.
When asked if he had ever used steroids, HGH or other performance-enhancing drugs, Rodriguez answered, "No."
"I got tested eight or nine times. Some of my teammates have been tested seven, eight or six times," Rodriguez added.
"I had heard that human growth hormone could promote faster healing for my elbow. I felt an obligation to get back to my team as soon as possible."
New York Yankees pitcher
"Bud Selig [Major League Baseball Commissioner] and Major League Baseball have done a fine job of implementing some very strict rules."
Pettitte and fellow pitcher Roger Clemens, who played for the Boston Red Sox, Houston Astros, Toronto Blue Jays and New York Yankees, were prominently mentioned in the Major League Baseball report on performance-enhancing drugs released on Thursday.
The report included information from Brian McNamee, former Yankees' trainer, who served as a personal trainer to both the Pettitte and Clemens up to the beginning of 2007.
McNamee recounts HGH use
"From April 21 to June 14, 2002, Pettitte was on the disabled list with elbow tendonitis," the Mitchell Report states.
"McNamee said that Pettitte called him while he was rehabilitating his elbow in Tampa, where the Yankees have a facility, and asked again about human growth hormone. Pettitte stated that he wanted to speed his recovery and help his team.
"McNamee traveled to Tampa at Pettitte's request and spent about 10 days assisting Pettitte with his rehabilitation.
"McNamee recalled that he injected Pettitte with human growth hormone that McNamee obtained from [Kirk] Radomski on two to four occasions. Pettitte paid McNamee for the trip and his expenses; there was no separate payment for the human growth hormone."
Pettitte comes clean
Pettitte came clean on Saturday, admitting that the Mitchell Report, as it pertained to him, was accurate.
"In 2002 I was injured," Pettitte said in a statement.
"I had heard that human growth hormone could promote faster healing for my elbow. I felt an obligation to get back to my team as soon as possible.
"For this reason, and only this reason, for two days I tried human growth hormone. Though it was not against baseball rules, I was not comfortable with what I was doing, so I stopped.
"This is it - two days out of my life; two days out of my entire career, when I was injured and on the disabled list."
Pettitte had a 15-9 win/loss record with a 4.05 earned run average (ERA) in 2007 and has a career 201-113 record with a 3.83 ERA in 13 seasons with the Yankees and the Houston Astros.