"We all wanted this story to come to an end, the wait was interminable, very frustrating and at times I thought the Tour de France had something personal against me," Pereiro told reporters at the headquarters of the Spanish Sports Council.
"I now realise the Tour organisers had to wait for a resolution. This is a very important day for me and I'm not going to ruin it by thinking about any appeal."
Landis, who finished 57 seconds ahead of Pereiro in the final standings, said last Wednesday he would appeal to the Court of Arbitration for Sport over the doping case which has also resulted in him receiving a two-year ban.
Pereiro held the yellow jersey for five days but was overhauled as leader when Landis staged an incredible comeback towards the end of the race.
It later emerged his former teammate had failed a doping test but runner-up Pereiro was not awarded victory until a lengthy investigation by the American Arbitration Association ended last month.
"It has been too long for Oscar Pereiro, his team and for Spain and too long for the Tour de France but the story has finally come to an end."
Tour de France director
"I feel sorry for him," said Pereiro.
"He was disqualified after the biggest triumph of his life and won't be able to enjoy it. But as a sportsman you have to stick to the rules."
Christian Prudhomme, Tour de France director, said Pereiro was the definitive winner.
"At last we have a winner. It has been too long for Oscar Pereiro, his team and for Spain and too long for the Tour de France but the story has finally come to an end," Prudhomme said.
"You can now take your place in the hall of fame alongside your illustrious countrymen who have won the Tour."
Sixth Spanish winner
Pereiro is the sixth Spaniard to win the Tour after Federico Bahamontes, Luis Ocana, Pedro Delgado, Miguel Indurain and 2007 winner Alberto Contador.
Jaime Lissavetzky, Spain's sports minister, said Landis's disqualification was a victory in the battle against doping.
"We need to separate those who play dirty from those who play clean," Lissavetzky said.
"We have been waiting to settle a debt with Oscar Pereiro and now it has been done.
"Cycling needs to hear some good news and this is a message that will help combat some of the disappointments it has endured."
Pereiro finished tenth in this year's Tour de France which was won by Contador, a surprise victor after leader Michael Rasmussen of Denmark was kicked out of the race in a row over missed drugs tests.