Fernandez de la Vega said Spain does "not support the policy of expulsions without respect for the law and rights, or actions which exalt violence, racism and xenophobia".
Berlusconi's government is preparing tougher measures against illegal immigrants, with the first expected to be approved at a cabinet meeting next week.
Cristian David, Romania's interior minister, meanwhile said on Friday that Romanians can circulate freely anywhere in the European Union.
"There are two things which are non-negotiable," David told a press conference in Bucharest. "One is respect for the law, and the other is respect for the right to free movement which Romanians acquired with European Union entry [on January 1, 2007]."
David was speaking following an emergency meeting in Rome the previous day with Roberto Maroni, his Italian counterpart, following the crackdown on immigrant crime.
Many see the crackdown as targeting Romanians.
"We fully support efforts by the Italian authorities towards a firm application of the law," David added.
"Romanians who live legally have nothing to fear," he underlined.
David also said that there was no general crime wave involving Romanians who have moved to Italy, adding that his country's people are contributors to Italy's national wealth.