Marcos will replace her son, Ferdinand Marcos Jr, in the lower house of parliament, while he seeks a senate seat.
Her eldest daughter, Imee, will be contesting the governorship of Ilocos Norte, the family's political stronghold.
It will be the first political outing for the former first lady since losing a presidential bid in 1992.
Marcos has said she hoped to achieve redemption for her husband, who is accused of stealing billions of dollars from state coffers during his 20-year rule, which ended with a "people power" revolution in 1986.
Famous for her flamboyant ways and a huge collection of shoes, Marcos returned to the Philippines from exile in 1983 and has emerged unscathed from corruption and human rights cases and enjoys a luxurious lifestyle in Manila.
Marcos received enthusiastic backing from Imee, who said her mother's experience and political savvy were the province's best bet.
"We need a real champion for the province because we are living in difficult times," she said.
"We must choose as our representative somebody who is intelligent, famous and influential."
Among other celebrities seeking election is Manny Pacquiao, the Filipino boxing star, who is seeking a congressional seat.
Gloria Macapagal Arroyo, the president, who has been threatened with criminal charges when her term ends in June, is also a candidate for the lower chamber.