Micheletti became the president of Honduras after Zelaya was ousted in a military-backed coup on June 28.
Since being removed, Zelaya tried twice to return to Honduras, but on both occasions the interim government foiled his attempts.
Connie Mack, a Republican member of the US house of representatives, told Reuters news agency he understood that two of the people who had their US visas revoked were Tomas Arita Valle, the supreme court justice who signed the order for Zelaya's arrest, and Jose Alfredo Saavedra, president of the Honduran congress.
Mack criticised the move as intimidation.
Two others who confirmed they had their visas revoked were Ramon Custodio, the human rights ombudsman, and Adolfo Lionel Sevilla, the defence minister in the interim government.
Micheletti told reporters at the presidential palace on Tuesday that his US visa had not been revoked.
Micheletti's government, which has the backing of the Honduran supreme court and congress, has refused to bend to international condemnation of the coup despite sanctions against it.
Washington has cut $16.5m in US military aid to the Central American country.
The EU has also suspended all budgetary support payments for Honduras.
The Inter-American Development Bank and the World Bank have frozen loans in a move the interim government says will cost $200m in 2009.
Zelaya has called for a ban on the coup leaders' bank transactions.