Las Cristinas mine, which is rich with gold, diamonds, iron and other minerals, is currently operated by Canadian firm Crystallex International Corp.
Crystallex issued a statement following the announcement saying they had "not been notified of any changes".
The company also said that Crystallex representatives had met Venezuelan officials on Wednesday and that "no issues were raised with regard to the control of Las Cristinas".
Chavez's government has clashed with other international gold-mining companies operating in Venezuela in recent months as it pursues pro-nationalisation policies.
Las Cristinas, thought to be venezuela's biggest gold mine, is located within the Imataca Forest Reserve, which is also home to unique plant and animal species.
Environmentalists have raised concerns that major mining operations could cause irreversible damange to the area's senstive ecosystem.