An opposition party co-ordinator in the southern town of Tataouine has died after his supporters clashed with those of the Islamist-led government, according to his Call of Tunisia party and the interior ministry.
A party official told the AFP news agency that Lotfi Naguedh died on Thursday afternoon after being beaten by pro-government demonstrators who attacked his office.
Khaled Tarrouche, an interior ministry spokesperson, confirmed the violence but said Naguedh had died of a heart attack.
A Call of Tunisia (Nida Touns in Arabic) spokesperson replied: "Even if it was a heart attack, it was directly linked to the attack."
Amateur video and witness accounts suggested violence on both sides, with supporters of Call of Tunisia throwing Molotov cocktails at supporters of the Ennahdha party.
Ennahdha, a centre-right Islamist party that is the strongest member of the ruling coalition, expressed its condolences to Naguedh's family in a statement emailed to Al Jazeera and called for a judicial inquiry into the incident.
The Call of Tunisia party was founded in June by Beji Caid Essebsi, a senior official in the first years of toppled president Zine El Abidine Ben Ali's rule, who served as interim prime minister in the wake of Ben Ali's overthrow last year.
The ruling coalition accuses him of regrouping former regime officials and seeking to undermine the government.
The League for the Protection of the Revolution, a grouping close to the ruling coalition, had called the demonstration on Thursday that then marched on the headquarters of a local trade union which Naguedh headed.
Outside the union building, the demonstration degenerated into clashes in which stones and Molotov cocktails were thrown by both sides, the interior ministry said.
"Police reinforcements were deployed and separated the two sides," the ministry spokesperson said. "The situation is now calm."
Call of Tunisia insisted the violence came solely from the pro-government demonstrators.
Senior party official Lazhar Lakremi condemned the "cowardly hand of treason and terrorism" behind Naguedh's death.
Last month, Lakremi charged that a member of the government, whom he did not identify, was plotting to assassinate the party leader Essebsi.
The League for the Protection of the Revolution has hit back with fiery rhetoric of its own, promising in an October 13 statement to make the "enemies of the revolution and henchmen of the old regime ... regret that they did not take their own lives".
The bad blood between the two sides has impacted on efforts to thrash out a post-revolution constitution so that a new date can be set for fresh elections that had been due to take place this year.
Two parties in the governing coalition - Ennahdha and the centre-left Congress for the Republic of President Moncef Marzouki - boycotted crisis talks on the new constitution on Tuesday to protest against the participation of Call of Tunisia.
They did so even though both leaders attended.