Raj Rajaratnam, the Sri Lankan-born billionaire and founder of the Galleon hedge fund, has been indicted in a US court on charges of securities fraud and conspiracy.
Rajaratnam and Danielle Chiesi, a former consultant to hedge fund New Castle LLC and a co-defendant in the case, were formally charged in an Manhattan federal court on Tuesday.
The case, which prosecutes have described as the biggest-ever hedge fund insider trading case, involved employees of some of America's best-known companies.
Rajaratnam's lawyer said he was innocent and would fight the charges.
"Mr Rajaratnam is innocent and looks forward to his day in court when a jury of his fellow citizens will examine and evaluate all of the evidence," John Dowd said in a statement after the indictment was returned.
Rajaratnam, a US citizen, was freed on $100m bail, an amount he hopes to have reduced.
Rajaratnam and Chiesi are the only indictments returned so far in the case, in which 20 people are facing criminal and civil charges.
Most of the accused have expertise in tech stocks.
The allegations included passing inside information on earnings announcements, takeovers and contracts on 10 companies, generating more than $30m in illegal profits, according to prosecutors.
Six traders or lawyers have pleaded guilty to charges in the investigation, which ensnared employees of IBM Corp, McKinsey & Co management and former lawyers of the Ropes & Gray law firm.
In the overall case, insider trading allegedly took place in shares of Google, Sun MicroSystems, Advanced Micro Devices, Polycom, Hilton Hotels, Intel, Clearwire, Akamai, Atheros and IBM among others.
Prosecutors say their investigation has identified about $40m in alleged illicit gains.
According to Forbes magazine, Rajaratnam is the world's 559th richest person with a net worth of $1.3bn.
His case has been followed closely in Sri Lanka, Rajaratnam's home country.