Waddah Khanfar, the director general of Aljazeera network, told AFP: "We do not have a political agenda against Tunisia or any other side.
"We regret the Tunisian decision, and we reaffirm that we are committed to [providing a platform for] different views".
Aljazeera would welcome "any Tunisian official who would want to speak to the channel," Khanfar said.
"When we host certain figures, it does not mean that Aljazeera endorses their positions," he said.
The Qatari government has not commented on Tunisia's decision to close its Doha embassy.
"When we host certain figures, it does not mean that Aljazeera endorses their positions"
Waddah Khanfar, chairman, Aljazeera network
Thursday's Qatari press reported the news but withheld editorial comment.
The Tunisian move followed the airing by Aljazeera of interviews with Moncef Marzouki, an opposition activist based in Paris who called for "civil resistance".
The Tunisian foreign ministry released a statement on Wednesday accusing Aljazeera of ignoring “truth and objectivity every time that it deals with current affairs in Tunisia ... apparently waging a hostile campaign aimed at harming Tunisia".