Batons and pepper spray were used by police, who numbered up to 200 on foot and 8 on horseback, to quell any trouble between the two groups of taunting fans.
The body armour clad officers cordoned off an estimated 400 England fans to keep them from engaging with several thousand German supporters who were celebrating their team's second round victory over Sweden.
England fans are in Stuttgart ahead of their side's second round match against Ecuador at the Gottlieb-Daimler Stadium on Sunday, with the winner going through to a quarter final match in Gelsenkirchen against either Portugal or the Netherlands.
By 8:30pm local time the situation had calmed down, although police maintained their presence in the area to prevent any further problems.
Mounted police try to calm
German fans in Stuttgart
This new instance of crowd trouble comes on the back of the brief detention of more than 120 England fans on Friday night after they caused a public nuisance through the streets of Stuttgart.