The news follows earlier speculation that he was set to make a dramatic comeback from injury.
British media, which has been following every detail of Rooney’s recovery after he fractured a bone in his right foot, had been reporting that the star striker would make an appearance from the substitutes bench in Thursday's game in Nuremberg.
After defeating Paraguay 1-0 on Saturday, a victory over Trinidad and Tobago would see England advance to the second round.
But reports on Tuesday said Rooney's return was unlikely as medical experts who said his foot injury had healed are not due to fly to Germany to re-assess the player's condition until Friday.
Manchester United manager Sir Alex Ferguson is reportedly furious with England counterpart Sven-Goran Eriksson over the Swede's refusal to rule Rooney out of the tournament until the second round.
United are concerned that Rooney has been rushed back too soon and risks further injury if he is forced to play without being properly match fit.
Rooney meanwhile has been training hard with the rest of the England team, although sources within the England camp have said coaching staff have asked Rooney to ease up amid worries he may injure himself again while attempting to prove his fitness to Eriksson.