The city, billed as the "biggest of its kind in the world," will host the premises of US universities that have opened branches in Qatar.
"The Amir will inaugurate the city on Monday," Saif al-Hajri, deputy board chairman of the Qatar Foundation for Education, Science and Community Development said.
The foundation is headed by the Amir's wife, Shaikha Mozah Bint Nasser al-Masnad, the driving force behind the project.
Hajri said the city was the costliest education project in the world, but did not give specific figures.
US universities that will have branches in the city include Cornell, Virginia Commonwealth and Texas A and M.
Hajri said leading European educational institutions will follow US counterparts in opening branches in the city, which will be developed over a decade.